Confirmation of Genome Editing Correction Rate Using NGS
The CRISPR-Cas system was initially studied as a bacterial defense mechanism against viral invasion. This system is now widely used for direct genome editing to study gene functions and enhance crop breeding by targeting specific genes.
To apply CRISPR-Cas technology, the first requirement is to have the sequence information of the specific gene, and secondly, the complete genomic sequence of the desired crop must be available. The editing of the targeted gene within the genome can be verified using NGS.
To apply CRISPR-Cas technology, the first requirement is to have the sequence information of the specific gene, and secondly, the complete genomic sequence of the desired crop must be available. The editing of the targeted gene within the genome can be verified using NGS.
Work Flow
Result Examples
The non-functional GFP gene sequence by Cas9/sgRNA-induced mutations (Jiang W, et al. 2013)