GBS analysis with a reference genome
GBS (Genotyping by Sequencing) utilizes NGS (Next Generation Sequencing) methods similar to Whole Genome Sequen`cing (WGS). Unlike WGS, which targets the entire genome, GBS involves creating a library from genomic DNA digested by a restriction enzyme. This approach specifically sequences fragments of defined sizes (200-500 bp), making it more cost-effective than other sequencing platforms like WGS.
When a reference genome of the target species is available, SNP (Single Nucleotide Polymorphisms) and small Insertions/Deletions (less than 50 bp) can be detected by aligning GBS reads against the reference genome.
When a reference genome of the target species is available, SNP (Single Nucleotide Polymorphisms) and small Insertions/Deletions (less than 50 bp) can be detected by aligning GBS reads against the reference genome.
Work Flow
Result Examples
An example of statistics of de-multiplexed GBS reads and alignment result
An example of SNP matrix displayed in Excel file format
Total of 118
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