GBS analysis without a reference genome
GBS (Genotyping by Sequencing) utilizes NGS (Next Generation Sequencing) methods similar to Whole Genome Sequencing (WGS). Unlike WGS, which targets the entire genome, GBS involves creating a library from genomic DNA digested by a restriction enzyme. This approach specifically sequences fragments of defined sizes (200-500 bp), making it more cost-effective than other sequencing platforms like WGS.
When a reference genome for the target species is unavailable, a draft genome construction of the genomic region or transcriptome through de novo assembly becomes essential to enhance mapping and genotyping accuracy. By aligning GBS (Genotyping by Sequencing) reads against this assembled draft genome, we can effectively detect SNP (Single Nucleotide Polymorphisms) and small Insertions/Deletions(less than 50 bp).
When a reference genome for the target species is unavailable, a draft genome construction of the genomic region or transcriptome through de novo assembly becomes essential to enhance mapping and genotyping accuracy. By aligning GBS (Genotyping by Sequencing) reads against this assembled draft genome, we can effectively detect SNP (Single Nucleotide Polymorphisms) and small Insertions/Deletions(less than 50 bp).
Work Flow
Result Examples
An example of statistics of draft genome assembly
An example of SNP matrix displayed in Excel file format
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